論文信息：Xiaoguo Zhu, Tuo Qi , Qian Yang, Fuxin He, Chenglong Tan, Wei Ma, Ralf Thomas Voegele, Zhensheng Kang, and Jun Guo. Host-induced gene silencing of the MAPKK gene PsFUZ7 confers stable resistance to wheat stripe rust. Plant Physiology(2017) DOI:10.1104/pp.17.01223
JCR分區(qū)Q1，IF=6.456
論文摘要：RNA interference (RNAi) is a powerful genetic tool to accelerate research in plant biotechnology and to control biotic stresses by manipulating target gene expression. However, the potential of RNAi in wheat to efficiently and durably control the devastating stripe rust fungus Puccinia striiformis f. sp. tritici (Pst), remained largely under explored, so far. To address this issue, we generated transgenic wheat lines expressing double-stranded RNA targeting PsFUZ7 transcripts of Pst. We analyzed expression of PsFUZ7 and related genes, and resistance traits of the transgenic wheat lines. We show that PsFUZ7 is an important pathogenicity factor which regulates infection and development of Pst. A PsFUZ7 RNAi construct stably expressed in two independent transgenic wheat lines confers strong resistance to Pst. Pst hyphal development is strongly restricted, and necrosis of plant cells in resistance responses was significantly induced. We conclude that trafficking of RNA molecules from wheat plants to Pst may lead to a complex molecular dialogue between wheat and the rust pathogen. Moreover, we confirm the RNAi-based crop protection approaches can be used as a novel control strategy against rust pathogens in wheat.
